Skip to Main content Skip to Navigation
Journal articles

High Dimensional Single-Cell Analysis Reveals iNKT Cell Developmental Trajectories and Effector Fate Decision

Abstract : CD1d-restricted invariant Natural Killer T (iNKT) cells represent a unique class of T lymphocytes endowed with potent regulatory and effector immune functions. Although these functions are acquired during thymic ontogeny, the sequence of events that gives rise to discrete effector subsets remains unclear. Using an unbiased single-cell transcriptomic analysis combined with functional assays, we reveal an unappreciated diversity among thymic iNKT cells, especially among iNKT1 cells. Mathematical modeling and biological methods unravel a developmental map whereby iNKT2 cells constitute a transient branching point toward the generation of iNKT1 and iNKT17 cells, which reconciles the two previously proposed models. In addition, we identify the transcription co-factor Four-and-a-half LIM domains protein 2 (FHL2) as a critical cell-intrinsic regulator of iNKT1 specification. Thus, these data illustrate the changing transcriptional network that guides iNKT cell effector fate.
Document type :
Journal articles
Complete list of metadata

https://hal-univ-tours.archives-ouvertes.fr/hal-03365048
Contributor : Christophe Paget Connect in order to contact the contributor
Submitted on : Tuesday, October 5, 2021 - 10:16:06 AM
Last modification on : Tuesday, October 19, 2021 - 11:56:32 PM

Links full text

Identifiers

Citation

Thomas Baranek, Kevin Lebrigand, Carolina de Amat Herbozo, Loïc Gonzalez, Gemma Bogard, et al.. High Dimensional Single-Cell Analysis Reveals iNKT Cell Developmental Trajectories and Effector Fate Decision. Cell Reports, Elsevier Inc, 2020, 32 (10), pp.108116. ⟨10.1016/j.celrep.2020.108116⟩. ⟨hal-03365048⟩

Share

Metrics

Record views

13