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, Each dot represents 765 the average of R for 10 z-stack images of a cell. Horizontal black bars stand for the mean value, 1NL4.3 with the PM probe dSQ12S was quantified with ImageJ software

. Mann-whitney, tests were performed to assess the significance of differences

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, Effect of the silencing of PIP5K1 isoforms on the rerouting of Pr55 Gag to early endosomes

, transfected with either a non-targeting control siRNA or with siRNAs 773 targeting each PIP5K1 isoform, were then transfected with plasmids encoding Pr55 Gag /Pr55 Gag-774 eGFP and mCh-Rab5. Z-stack confocal images were acquired from living cells as previously 775 described. Representative yellow merged images (green for Pr55 Gag and red for mCh-Rab5) are 776 shown for cells transfected with non-targeting control siRNA or siRNA targeting PIP5K1?, TZM-bl HeLa cells, p.777

, PIP5K1? or PIP5K1? (a, b, c and d, respectively). (B) The colocalization of Pr55 Gag-eGFP with 778 mCh-Rab5 was quantified with ImageJ software. Each dots represents the average of R for, vol.10, p.779