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Role of chromatin structure in transcriptional regulation of MMTV LTR hormone-dependent promoter.

Abstract : Studies of chromatin structure were performed in mouse fibroblast cell lines containing Bovine Papilloma Virus (BPV) based artificial minichromosomes containing Mouse Mammary Tumor Virus (MMTV) Long Terminal Repeat (LTR), a retroviral promoter regulated by glucocorticoids, driving the transcription of v-Ha-ras. These minichromosomes fractionate with the "active chromatin", indicating an association of the minichromosomes with components of the "nuclear matrix". Two regions of the minichromosomes upstream and downstream of v-Ha-ras are involved in this interaction. MMTV LTR promoter is associated with nucleosomes precisely positioned on the DNA sequences. Hormonal activation is accompanied by a structural change of the nucleosome associated with the hormone response elements (HREs). This structural change can be visualized by the appearance of a hormono-dependent DNaseI hypersensitive site. Anti-hormones, even when able to promote a strong binding of the receptor to the nucleus, are unable to induce the chromatin structural change. The strong association of the hormone-receptor complex with the nucleus is necessary to induce the DNaseI hypersensitive site and to maintain the transcription, but is not necessary for DNaseI hypersensitivity maintenance. This suggests a double role for the hormone-receptor complex: 1) induction of a chromatin rearrangement and 2) transcriptional transactivation.
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Contributor : Fabrice Gouilleux Connect in order to contact the contributor
Submitted on : Saturday, January 4, 2020 - 10:04:50 AM
Last modification on : Monday, July 4, 2022 - 10:02:46 AM


  • HAL Id : hal-02427761, version 1
  • PUBMED : 2168572


H. Richard-Foy, J Adom, K Carr, F. Gouilleux, V. Marsaud, et al.. Role of chromatin structure in transcriptional regulation of MMTV LTR hormone-dependent promoter.. Revista espanola de fisiologia, 1990, 46 (1), pp.31-7. ⟨hal-02427761⟩



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