Colony-stimulating factors and interferon-γ activate a protein related to MGF-Stat 5 to cause formation of the differentiation-induced factor in myeloid cells - Archive ouverte HAL Access content directly
Journal Articles FEBS Letters Year : 1995

Colony-stimulating factors and interferon-γ activate a protein related to MGF-Stat 5 to cause formation of the differentiation-induced factor in myeloid cells

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Fariba Barahmand-Pour
  • Function : Author
Andreas Meinke
  • Function : Author
Andreas Eilers
  • Function : Author
Bernd Groner
  • Function : Author
Thomas Decker
  • Function : Author
  • PersonId : 1015188

Abstract

The Jak-Stat pathway of intracellular signals is used by growth factor- and cytokine receptors to induce gene transcription. We have recently reported that differentiation of myeloid cells, induced by phorbol ester, interferon-gamma (IFN-gamma) or colony-stimulating factor-1 (CSF-1) is accompanied by the activation of the differentiation-induced factor (DIF). Activated DIF specifically associates with a subclass of gamma-interferon activation site (GAS)-like DNA elements. We now report that GM-CSF, which like CSF-1 promotes the generation of mature macrophages, activates DIF. No activation was observed after treatment with the granulocyte growth and differentiation factor G-CSF. Antibodies raised against a Stat family protein, designated mammary gland factor-Stat 5 (MGF-Stat 5), reacted with DIF induced by either CSF-1, GM-CSF or IFN-gamma. Antisera to other known Stats were without effect on the DIF complex in electrophoretic mobility shift assays (EMSA). A 112 kDa protein could be isolated from either GM-CSF- or IFN-gamma-treated cells by GAS oligonucleotide precipitation. This protein reacted with antibodies to both MGF-Stat 5 and phosphotyrosine. MGF-Stat 5 and closely related proteins thus define a subfamily of Stat transcription factors that are present in a variety of cell types and are required for the onset of immediate gene expression in response to differentiating stimuli.
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hal-02427586 , version 1 (04-11-2021)

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Fariba Barahmand-Pour, Andreas Meinke, Andreas Eilers, Fabrice Gouilleux, Bernd Groner, et al.. Colony-stimulating factors and interferon-γ activate a protein related to MGF-Stat 5 to cause formation of the differentiation-induced factor in myeloid cells. FEBS Letters, 1995, 360 (1), pp.29-33. ⟨10.1016/0014-5793(95)00072-h⟩. ⟨hal-02427586⟩

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