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Interleukin-3, Erythropoietin, and Prolactin Activate a STAT5-like Factor in Lymphoid Cells

Abstract : Interleukin-3 (IL-3)-, erythropoietin (EPO)-, and prolactin (PRL)-induced signal transduction via the JAK/STAT pathway was studied in the IL-3-dependent BAF-3 lymphoid cell line. Transfected cells expressing either the long form of the PRL receptor or the EPO receptor were used. We demonstrated that IL-3, EPO, and PRL activated a transcription factor related to the mammary transcription factor STAT5 but not to STAT1, -2, -3, or -4 as opposed to interferon gamma (IFN gamma) which activated STAT1 in the same cells. Similarly, PRL and EPO activated a STAT5-like factor (STAT5-L) in the rat Nb2 and the human UT7 cells expressing endogenous PRL and EPO receptors, respectively. The hematopoietic STAT5-L activated by IL-3, EPO, or PRL was identified as a 97-kDa tyrosine-phosphorylated protein. These results confer to STAT5 a much broader role than previously suggested.
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https://hal-univ-tours.archives-ouvertes.fr/hal-02427576
Contributor : Fabrice Gouilleux <>
Submitted on : Friday, January 3, 2020 - 5:35:36 PM
Last modification on : Monday, January 6, 2020 - 4:30:25 PM

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C. Pallard, F. Gouilleux, M. Charon, B. Groner, S. Gisselbrecht, et al.. Interleukin-3, Erythropoietin, and Prolactin Activate a STAT5-like Factor in Lymphoid Cells. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 1995, 270 (27), pp.15942-15945. ⟨10.1074/jbc.270.27.15942⟩. ⟨hal-02427576⟩

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