Prolactin and interleukin-2 receptors in T lymphocytes signal through a MGF-STAT5-like transcription factor.

F. Gouilleux (1) , D Moritz , M Humar , R Moriggl , S Berchtold , B Groner

1 GICC UMR 6239 CNRS - Génétique, immunothérapie, chimie et cancer (GICC), UMR 6239 CNRS [2008-2011]

The cell surface receptors for PRL and interleukin-2 (IL-2) are structurally distinct, but share regulatory tasks in T lymphocytes. They can stimulate proliferation and activate transcription of over-lapping sets of genes of T cells. PRL and IL-2 receptor activation are both linked to the Jak/Stat (signal transducer and activator of transcription) pathway. We investigated the ability of PRL and IL-2 to activate Stat proteins in different T cell lines. The DNA binding specificities, the reactivities toward Stat-specific antisera, and the mol wt of IL-2- and PRL-induced DNA-binding proteins in Nb2 and C196 T cell lines were investigated. A comparison with the Stat proteins induced by interferon-gamma, PRL, and IL-6 in T47D mammary tumor cells was made. We found that these parameters were indistinguishable for one of the PRL- and IL-2-induced factors. A transcription factor closely related to mammary gland factor-Stat5 is rapidly activated upon interaction of IL-2 and PRL with their respective receptors. Activation of a second protein related to Stat1 was also observed. Our results emphasize the role of PRL as a regulator of the immune response and indicate that the Stat factors mammary gland factor-Stat5 and Stat1 play a role in the regulation of gene expression during T cell development.

Domaines: Sciences du Vivant [q-bio]

F. Gouilleux, D Moritz, M Humar, R Moriggl, S Berchtold, et al.. Prolactin and interleukin-2 receptors in T lymphocytes signal through a MGF-STAT5-like transcription factor.. Endocrinology, 1995, 136 (12), pp.5700-5708. ⟨10.1210/endo.136.12.7588326⟩. ⟨hal-02427567⟩