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Modulation of the activation status of Stat5a during LIF-induced differentiation of M1 myeloid leukemia cells

Abstract : Treatment of M1 myeloid leukemia cells with leukemia inhibitory factor (LIF) causes activation of transcription factors Stat1, Stat3 and Stat5a (signal transducers and activators of transcription). DNA-binding of Stat proteins was detectable for extended periods of time in LIF-treated M1 cells, which simultaneously underwent terminal differentiation. The relative composition of Stat factors in the protein-DNA complexes changed during time. Whereas Stat3 was activated up to 36 h during treatment with LIF, Stat5a was activated only short-termed. Similarly, high expression of the immediate early gene CIS (cytokine-inducible SH2-containing protein), a known target gene of Stat5 in hematopoietic cells, occurred only during the onset of differentiation. This suggests a role of Stat5a in the early phase of LIF-induced differentiation and growth arrest of M1 cells.
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https://hal-univ-tours.archives-ouvertes.fr/hal-02427392
Contributor : Fabrice Gouilleux <>
Submitted on : Friday, January 3, 2020 - 3:26:02 PM
Last modification on : Monday, January 6, 2020 - 4:14:36 PM

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Roland Piekorz, Ralf Rinke, Fabrice Gouilleux, Brigitte Neumann, Bernd Groner, et al.. Modulation of the activation status of Stat5a during LIF-induced differentiation of M1 myeloid leukemia cells. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1998, 1402 (3), pp.313-323. ⟨10.1016/s0167-4889(98)00024-x⟩. ⟨hal-02427392⟩

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