Skip to Main content Skip to Navigation
Journal articles

A Single Amino Acid in the DNA Binding Regions of STAT5A and STAT5B Confers Distinct DNA Binding Specificities

Abstract : STAT5A and STAT5B are two highly related transcription factors encoded by two distinct genes. STAT5A and STAT5B are activated by a broad range of cytokines and growth factors. Although they can be differentially activated, the functional difference between these two molecules relative to their structure is not known. Here we demonstrated that STAT5A and STAT5B homodimers have distinct DNA binding preferences. Chimeric STAT5 molecules allowed us to identify a region between amino acid 420 and 545 responsible for the DNA binding specificity. This region is located in the previously characterized DNA binding region of STAT proteins. Sequence comparison between STAT5A and STAT5B from different species showed a difference of 5 amino acids in the region 420-545 between STAT5A and STAT5B. Substitution of these amino acids demonstrated that a glycine residue at position 433 in STAT5B and a glutamic residue at a similar position in STAT5A determined the DNA binding specificity. These data indicate that STAT5A and STAT5B homodimers may have distinct function and probably regulate the expression of common as well as distinct genes.
Document type :
Journal articles
Complete list of metadatas

https://hal-univ-tours.archives-ouvertes.fr/hal-02427387
Contributor : Fabrice Gouilleux <>
Submitted on : Friday, January 3, 2020 - 3:24:15 PM
Last modification on : Thursday, April 9, 2020 - 11:56:35 AM

Links full text

Identifiers

Citation

Christine Boucheron, Stephanie Dumon, Susana Constantino Rosa Santos, Richard Moriggl, Lothar Hennighausen, et al.. A Single Amino Acid in the DNA Binding Regions of STAT5A and STAT5B Confers Distinct DNA Binding Specificities. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 1998, 273 (51), pp.33936-33941. ⟨10.1074/jbc.273.51.33936⟩. ⟨hal-02427387⟩

Share

Metrics

Record views

313