Interleukin-7 induces apoptosis of 697 pre-B cells expressing dominant-negative forms of STAT5: evidence for caspase-dependent and -independent mechanisms - Archive ouverte HAL Access content directly
Journal Articles Oncogene Year : 2004

Interleukin-7 induces apoptosis of 697 pre-B cells expressing dominant-negative forms of STAT5: evidence for caspase-dependent and -independent mechanisms

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1
Olivia Lanvin
  • Function : Author
Fabrice Gouilleux
Catherine Mullié
Cécile Mazière
  • Function : Author
  • PersonId : 910165
Vincent Fuentes
  • Function : Author
Eliane Bissac
  • Function : Author
Françoise Dantin
  • Function : Author
Jean-Claude Maziere
  • Function : Author
  • PersonId : 918627
  • IdRef : 111284570
Aline Regnier
  • Function : Author
Kaiss Lassoued
  • Function : Author
Valérie Gouilleux-Gruart

Abstract

The transcription factors STAT5A and STAT5B (STAT: signal transducer and activator of transcription) play a major role in the signaling events elicited by a number of growth factor and cytokine receptors. In this work, we aimed to investigate the role of STAT5 in human precursor B cell survival by introducing dominant-negative (DN) forms of STAT5A or STAT5B in the 697 pre-B cell line. All clones expressing DN forms of either transcription factor exhibited a higher spontaneous apoptotic rate that was massively enhanced upon interleukin-7 (IL-7) stimulation. This was associated with caspase 8 cleavage, mitochondrial transmembrane potential disruption and caspase 3 activation. However, the DN forms of STAT5 did not alter the expression of Bcl-2, Bax, Bcl-x, Bim, A1 and Mcl1 proteins in IL-7-stimulated cells. The pancaspase inhibitor Z-Val-Ala-Asp-fluoromylmethyl ketone partially suppressed IL-7-mediated mitochondrial transmembrane potential disruption and cell death, suggesting that IL-7 induced the death of DN STAT5 expressing 697 cells through caspase-dependent and -independent mechanisms that both require mitochondrial activation.
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hal-02425322 , version 1 (29-10-2021)

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Olivia Lanvin, Fabrice Gouilleux, Catherine Mullié, Cécile Mazière, Vincent Fuentes, et al.. Interleukin-7 induces apoptosis of 697 pre-B cells expressing dominant-negative forms of STAT5: evidence for caspase-dependent and -independent mechanisms. Oncogene, 2004, 23 (17), pp.3040-3047. ⟨10.1038/sj.onc.1207450⟩. ⟨hal-02425322⟩

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