Evidence for glycosylphosphatidylinositol (GPI)-anchored eosinophil-derived neurotoxin (EDN) on human granulocytes

Françoise Debierre-Grockiego (1) , Corinne Desaint (2) , Vincent Fuentes , Mathilde Poussin , Gérard Socié (3) , Nahid Azzouz (4) , Ralph Schwarz , Lionel Prin (5) , Valérie Gouilleux-Gruart (6)

1 Institut für Virologie
2 CIC 1417 - CIC Cochin Pasteur
3 Unité d'hématologie et de transplantation
4 Institute for Virology, Laboratory of Parasitology
5 Inflammation: mécanismes et régulation et interactions avec la nutrition et les candidoses
6 Développement normal et pathologique des lymphocytes et signalisation

Eosinophil-derived neurotoxin (EDN) is one of the four basic proteins stored in specific eosinophil granules. Here we demonstrate that EDN can also be detected at the surface of granulocytes. Reduction of EDN membrane expression after phosphatidylinositol-specific phospholipase C treatment suggests that a glycosylphosphatidylinositol (GPI) anchor is involved in the membrane association of EDN. The presence of a GPI anchor was confirmed by a lower expression of membrane EDN on granulocytes from patients with paroxysmal nocturnal hemoglobinuria which present cells lacking GPI anchor proteins. Furthermore, metabolic labeling with GPI anchor components supports biochemical evidence of GPI anchoring of EDN.

Domaines: Sciences du Vivant [q-bio]

Françoise Debierre-Grockiego, Corinne Desaint, Vincent Fuentes, Mathilde Poussin, Gérard Socié, et al.. Evidence for glycosylphosphatidylinositol (GPI)-anchored eosinophil-derived neurotoxin (EDN) on human granulocytes. FEBS Letters, 2003, 537 (1-3), pp.111-116. ⟨10.1016/s0014-5793(03)00106-6⟩. ⟨hal-02425315⟩