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Evidence for glycosylphosphatidylinositol (GPI)-anchored eosinophil-derived neurotoxin (EDN) on human granulocytes

Abstract : Eosinophil-derived neurotoxin (EDN) is one of the four basic proteins stored in specific eosinophil granules. Here we demonstrate that EDN can also be detected at the surface of granulocytes. Reduction of EDN membrane expression after phosphatidylinositol-specific phospholipase C treatment suggests that a glycosylphosphatidylinositol (GPI) anchor is involved in the membrane association of EDN. The presence of a GPI anchor was confirmed by a lower expression of membrane EDN on granulocytes from patients with paroxysmal nocturnal hemoglobinuria which present cells lacking GPI anchor proteins. Furthermore, metabolic labeling with GPI anchor components supports biochemical evidence of GPI anchoring of EDN.
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Contributor : Valérie Gouilleux Connect in order to contact the contributor
Submitted on : Monday, December 30, 2019 - 12:05:59 PM
Last modification on : Friday, August 5, 2022 - 11:21:53 AM

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Françoise Debierre-Grockiego, Corinne Desaint, Vincent Fuentes, Mathilde Poussin, Gérard Socié, et al.. Evidence for glycosylphosphatidylinositol (GPI)-anchored eosinophil-derived neurotoxin (EDN) on human granulocytes. FEBS Letters, Wiley, 2003, 537 (1-3), pp.111-116. ⟨10.1016/s0014-5793(03)00106-6⟩. ⟨hal-02425315⟩



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