Evidence for glycosylphosphatidylinositol (GPI)-anchored eosinophil-derived neurotoxin (EDN) on human granulocytes - Archive ouverte HAL Access content directly
Journal Articles FEBS Letters Year : 2003

Evidence for glycosylphosphatidylinositol (GPI)-anchored eosinophil-derived neurotoxin (EDN) on human granulocytes

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Abstract

Eosinophil-derived neurotoxin (EDN) is one of the four basic proteins stored in specific eosinophil granules. Here we demonstrate that EDN can also be detected at the surface of granulocytes. Reduction of EDN membrane expression after phosphatidylinositol-specific phospholipase C treatment suggests that a glycosylphosphatidylinositol (GPI) anchor is involved in the membrane association of EDN. The presence of a GPI anchor was confirmed by a lower expression of membrane EDN on granulocytes from patients with paroxysmal nocturnal hemoglobinuria which present cells lacking GPI anchor proteins. Furthermore, metabolic labeling with GPI anchor components supports biochemical evidence of GPI anchoring of EDN.

Dates and versions

hal-02425315 , version 1 (30-12-2019)

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Cite

Françoise Debierre-Grockiego, Corinne Desaint, Vincent Fuentes, Mathilde Poussin, Gérard Socié, et al.. Evidence for glycosylphosphatidylinositol (GPI)-anchored eosinophil-derived neurotoxin (EDN) on human granulocytes. FEBS Letters, 2003, 537 (1-3), pp.111-116. ⟨10.1016/s0014-5793(03)00106-6⟩. ⟨hal-02425315⟩
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