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IL-2 Phosphorylates STAT5 To Drive IFN-γ Production and Activation of Human Dendritic Cells

Abstract : Human dendritic cells (hDCs) produce IL-2 and express IL-2R α-chain (CD25), but the role of IL-2 in DC functions is not well defined. A recent study suggested that the main function of CD25 on hDCs was to transpresent IL-2 to activate T lymphocytes. Our results demonstrate the expression of the three chains of the IL-2R on hDCs and that IL-2 induces STAT5 phosphorylation. Interestingly, use of inhibitors of p-STAT5 revealed that IL-2 increases LPS-induced IFN-γ through STAT5 phosphorylation. Finally, we report that IL-2 increases the ability of hDCs to activate helpless CD8+ T cells, most likely because of IL-2–triggered IFN-γ synthesis, as we previously described. For the first time, to our knowledge, we disclose that IL-2 induces monocyte-derived hDC's functional maturation and activation through IL-2R binding. Interestingly, our study suggests a direct effect of anti-CD25 mAbs on hDCs that may contribute to their clinical efficacy.
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https://hal-univ-tours.archives-ouvertes.fr/hal-02423563
Contributor : Fabrice Gouilleux Connect in order to contact the contributor
Submitted on : Saturday, October 30, 2021 - 10:08:35 AM
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Florence Herr, Roxane Lemoine, Fabrice Gouilleux, Daniel Meley, Ihab Kazma, et al.. IL-2 Phosphorylates STAT5 To Drive IFN-γ Production and Activation of Human Dendritic Cells. Journal of Immunology, Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists, 2014, 192 (12), pp.5660-5670. ⟨10.4049/jimmunol.1300422⟩. ⟨hal-02423563⟩

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