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IgE receptor on human eosinophils (FcERII). Comparison with B cell CD23 and association with an adhesion molecule.

Abstract : IgE FcR (FcERII) on human eosinophils was characterized and compared with FcERII present on B cells (CD23). Two mAb, BB10 (anti-eosinophil FcERII) and 135 (anti-CD23), bound to the major component of FcERII at 45,000 to 50,000 Mr, both on purified hypodense eosinophils and on a B cell line (WIL-2WT). The specific ligand, human myeloma IgE, was able to bind to the molecules immunoprecipitated by BB10. A cross-reactivity between BB10 and a mAb anti-Leishmania gp63, which is a "fibronectin (Fn)-like" molecule, containing the L-arginine-L-glycyl-L-aspartyl (RGD) cell attachment domain indicated the presence of such a sequence in the common structure present on eosinophil and B cell FcERII. The synthetic tetrapeptide RGDS as well as its inverted sequence (SDGR) reduced the binding of BB10 and anti-Fn mAb to eosinophils and B cells. Flow microfluorometry analysis revealed a variable binding of BB10 and anti-Fn mAb to eosinophils purified from different patients, results compatible with recent findings on the inducibility of FcERIIb. The significant inhibition of IgE-dependent cytotoxicity against parasite targets by preincubation of eosinophils with BB10, anti-Fn and anti-CD23 mAb, with anti-RGDS polyclonal antibodies or with the SDGR peptide suggested the requirement of this cell adhesion sequence for the function of low affinity FcERII. The presence of such a sequence in the C-terminal domain of B cell FcERII raised the possibility of its role in B cell adhesion or B cell growth.
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Journal articles
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https://hal-univ-tours.archives-ouvertes.fr/hal-02414872
Contributor : Valérie Gouilleux <>
Submitted on : Monday, December 16, 2019 - 6:26:02 PM
Last modification on : Friday, January 10, 2020 - 4:12:10 PM

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  • HAL Id : hal-02414872, version 1
  • PUBMED : 2531185

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C Grangette, V Gruart, M. Ouaïssi, F Rizvi, G Delespesse, et al.. IgE receptor on human eosinophils (FcERII). Comparison with B cell CD23 and association with an adhesion molecule.. Journal of Immunology, Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists, 1989, 143 (11), pp.3580-8. ⟨hal-02414872⟩

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