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Heterogeneous expression of CD23 epitopes by eosinophils from patients. Relationships with IgE-mediated functions

Abstract : The receptor for IgE (Fc epsilon RII) on human eosinophils presents some common characteristics with CD23, a differentiation marker of B cells. We have used flow cytometry for evaluating the expression of various epitopes of CD23 on purified eosinophils from patients with eosinophilia. A correlation was found between the binding of myeloma IgE protein and the binding of a monoclonal antibody (mAb 135), directed against the IgE-binding site of B cell CD23. Using two additional anti-CD23 mAb, directed (8-30) or not (3-5) against the IgE-binding site, a low expression of these CD23 epitopes was observed on eosinophils from different eosinophilic patients. Northern blot analysis of eosinophil RNA with the cDNA probe of CD23 revealed a low-abundance transcript in three of the six patients expressing membrane CD23. The inhibition by all anti-CD23 mAb of IgE-mediated cytotoxicity and IgE binding to eosinophils clearly indicated the participation of a CD23-related molecule in IgE-dependent eosinophil functions.
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https://hal-univ-tours.archives-ouvertes.fr/hal-02414808
Contributor : Valérie Gouilleux <>
Submitted on : Monday, December 16, 2019 - 5:52:46 PM
Last modification on : Friday, January 10, 2020 - 4:12:10 PM

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Monique Capron, Marie-José Truong, Delphine Aldebert, V Gruart, Masaki Suemura, et al.. Heterogeneous expression of CD23 epitopes by eosinophils from patients. Relationships with IgE-mediated functions. European Journal of Immunology, Wiley-VCH Verlag, 1991, 21 (10), pp.2423-2429. ⟨10.1002/eji.1830211019⟩. ⟨hal-02414808⟩

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