Impact of Site-Specific Conjugation of ScFv to Multifunctional Nanomedicines Using Second Generation Maleimide

Katel Hervé-Aubert 1, * Emilie Allard-Vannier 1 Nicolas Joubert 2 Zineb Lakhrif 3 Christophe Alric 1 Camille Martin 2 Marie-Claude Viaud-Massuard 2 Isabelle Dimier-Poisson 3 Nicolas Aubrey 3 Igor Chourpa 1
* Corresponding author
1 NMNS - Nanomédicaments et Nanosondes, EA 6295
2 GICC - Génétique, Immunothérapie, Chimie et Cancer
3 INRA - U. Tours, UMR 1282 ISP Infectiologie et Santé Publique
Abstract : Biocompatible multifunctional nanomedicines (NMs) are known to be an attractive platform for targeted anticancer theranosis. However, these nanomedicines are of interest only if they efficiently target diseased cells and accumulate in tumors. Here we report the synthesis of a new generation of immunotargeted nanomedicines composed of a superparamagnetic iron oxide nanoparticle (SPION) core, polyethylene glycol coating and the anti-HER2 single chain fragment variable (scFv) of Trastuzumab antibody. We developed two novel bioengineered scFv carrying two cysteines located (i) at the end (4D5.1-cys2) or (ii) at the beginning (4D5.2-cys2) of its hexahistidine tag. The scFv bioconjugation was controlled via heterobifunctional linkers including a second generation maleimide (SGM). Our data indicated that the insertion of cysteines at the beginning of the hexahistidine tag was allowed to obtain nearly 2-fold conjugation efficiency (13 scFv/NP) compared to NMs using classical maleimide. As a result, the NMs-4D5.2 built using the optimal 4D5-cys2 and linkers equipped with SGM showed the enhanced recognition of HER2 in an ELISA format and on the surface of SK-BR-3 breast cancer cells in vitro. Their stability in serum was also significantly improved compared to the NMs-4D5. Our results showed the fundamental importance of the controlled ligand conjugation in the perspective of rational design of NMs with tailored physicochemical and biological properties.
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https://hal-univ-tours.archives-ouvertes.fr/hal-01757120
Contributor : Martin Soucé <>
Submitted on : Tuesday, April 3, 2018 - 2:08:42 PM
Last modification on : Wednesday, April 4, 2018 - 1:02:18 AM

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Katel Hervé-Aubert, Emilie Allard-Vannier, Nicolas Joubert, Zineb Lakhrif, Christophe Alric, et al.. Impact of Site-Specific Conjugation of ScFv to Multifunctional Nanomedicines Using Second Generation Maleimide. Bioconjugate Chemistry, American Chemical Society, 2018, ⟨10.1021/acs.bioconjchem.8b00091⟩. ⟨hal-01757120⟩

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