Confocal Raman spectroscopic imaging for in vitro monitoring of active ingredient penetration and distribution in Reconstructed Human Epidermis model - Université de Tours Accéder directement au contenu
Article Dans Une Revue Journal of Biophotonics Année : 2017

Confocal Raman spectroscopic imaging for in vitro monitoring of active ingredient penetration and distribution in Reconstructed Human Epidermis model

Résumé

Topically applied active cosmetic ingredients (ACI) or active pharmaceutical ingredients (API) efficacy is directly related to their efficiency of penetration in the skin. In vitro reconstructed human epidermis surrogate models offer in vivo like skin samples for transdermal studies. Using Delipidol®, an ACI currently used in the cosmetics industry, the capabilities to deliver accurate distribution maps and penetration profiles of this molecule by means of confocal Raman spectroscopic imaging have been demonstrated. Using a non-negative constrained least squares (NCLS) approach, contribution of specific molecules can be estimated at each point of spectral maps in order to deliver semi-quantitative heat maps representing the ACI levels in the different skin layers. The concentration profiles obtained are approximately single exponential for all 3 time points evaluated, with a consistent decay constant, which is independent of the sublayer structure. Notably, however, there is no significant penetration into the lower basal layers until a critical concentration is built up, after 3 hours. Combination of Raman confocal imaging with spectral unmixing methods such as NCLS is demonstrated to be a relevant approach for in vitro biological evaluation of cosmetic and pharmaceutical active ingredients and could easily be implemented as a screening tool for industrial use.

Dates et versions

hal-01643896 , version 1 (21-11-2017)

Identifiants

Citer

Lynda Miloudi, Franck Bonnier, Ali Tfayli, Florent Yvergnaux, Hugh J. Byrne, et al.. Confocal Raman spectroscopic imaging for in vitro monitoring of active ingredient penetration and distribution in Reconstructed Human Epidermis model. Journal of Biophotonics, 2017, pp.e201700221. ⟨10.1002/jbio.201700221⟩. ⟨hal-01643896⟩
122 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More