Covalent conjugation of cysteine-engineered scFv to PEGylated magnetic nanoprobes for immunotargeting of breast cancer cells

Christophe Alric 1 Nicolas Aubrey 2 Émilie Allard-Vannier 1 Anne Di Tommaso 2 Thibaut Blondy 1 Isabelle Dimier-Poisson 2 Igor Chourpa 1 Katel Hervé-Aubert 1, *
* Corresponding author
1 NMNS - Nanomédicaments et Nanosondes, EA 6295
2 UR IASP - UR Infectiologie animale et Santé publique
Abstract : In the present study, we describe the synthesis and characterization of new generation of cancer-targeting magnetic nanoprobes: superparamagnetic iron oxide nanoparticles (SPIONs) coated with polyethylene glycol (PEG) shell functionalized with recombinant anti-HER2 single chain fragment variable (scFv) of Trastuzumab antibody. An anti-HER2 scFv with terminal cysteine (scFv 4D5-Cys) has been rationally engineered in order to favor its orientation- and site-directed covalent conjugation to the polymeric surface of PEGylated SPIONs. Optimization of scFv and nanoparticles production allowed to obtain well-characterized SPIONs-PEG–scFv nanoparticles carrying ∼7 fragments per nanoparticle, having a hydrodynamic diameter of ca. 86 nm and nearly neutral surface. The nanoprobes-scFv capability to recognize the HER2 protein has been confirmed by enzyme-linked immunosorbent assay (ELISA). Compared to non-targeted PEGylated SPIONs, the SPIONs–PEG–scFv nanoprobes showed an enhanced binding to HER2-overexpressing cells (SK-BR3) in vitro as it was shown by immunofluorescence. Finally, ICP-AES measurements shown that in 1 hour the uptake of SPIONs–PEG–scFv in HER2-overexpressing cells is 2.1 times greater than non-targeted PEGylated SPIONs. Therefore, both due to their physico-chemical characteristics and the immunotargeting of HER2-positive breast cancer cells, the SPIONs–PEG–scFv appear as promising nanoplatforms for future applications in theranostic treatment of cancers.
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Submitted on : Friday, April 29, 2016 - 4:20:29 PM
Last modification on : Wednesday, March 21, 2018 - 10:54:10 AM

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Christophe Alric, Nicolas Aubrey, Émilie Allard-Vannier, Anne Di Tommaso, Thibaut Blondy, et al.. Covalent conjugation of cysteine-engineered scFv to PEGylated magnetic nanoprobes for immunotargeting of breast cancer cells. RSC Advances, Royal Society of Chemistry, 2016, 6 (43), pp.37099-37109. ⟨10.1039/c6ra06076e⟩. ⟨hal-01309513⟩

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