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Journal articles
Sensitive trimodal MRI-SERRS-fluorescence detection of cancer cells with stable magneto-plasmonic nanoprobes
Abstract : Novel magneto-plasmonic nanoprobes were designed for multimodal diagnosis of cancer by
combination of Magnetic Resonance Imaging (MRI), Surface-Enhanced Resonance Raman
Scattering (SERRS) and fluorescence emission in the very near infrared (VNIR). A controlled
electrostatic assembly of silver nanoparticles (Ag NPs), superparamagnetic iron oxide
nanoparticles (SPIONs), VNIR dye Nile blue (NB) and biopolymer chitosan (Chi) was used to
formulate the AgIONs-Chi nanoprobes. The formulation protocol did not involve organic
solvents, was rapid and efficient as confirmed by magnetic sorting. The SERRS response of the
nanoprobes was very intense and constant for days. It decreased linearly upon 1000-fold dilution
and was still recognizable at 0.1 nM NB concentration. After 30 days storage, the SERRS loss
was less than 30% and the hydrodynamic size of the AgIONs-Chi in PBS remained below
200nm. The gradual decrease of the ratio SERRS/fluorescence allowed monitoring the release of
the fluorescent molecule upon long-term nanoprobe dissociation. The AgIONs-Chi exhibited
two-fold higher MRI contrast than that of commercially available SPION suspensions. Finally,
the nanoprobes were actively uptaken by HeLa cancer cells and ensured trimodal MRI-SERRSfluorescence
detection of 10μL cell inclusions in cm-sized agarose gels used here as phantom
models of microtumors. The above results show that the magneto-plasmonic AgIONs-Chi are
promising substrates for SERRS analysis in solution and for multimodal imaging of cancer cells.
https://hal-univ-tours.archives-ouvertes.fr/hal-01220301
Contributor : Martin Soucé <>
Submitted on : Monday, October 26, 2015 - 9:57:00 AM
Last modification on : Tuesday, March 23, 2021 - 5:12:07 PM
Contributor : Martin Soucé <>
Submitted on : Monday, October 26, 2015 - 9:57:00 AM
Last modification on : Tuesday, March 23, 2021 - 5:12:07 PM