Mec1-Dependent Phosphorylation of the Scc3 Subunit of Cohesin during Mitosis in Budding Yeast

Abstract : Cohesin is an evolutionary conserved complex that controls chromosome segregation during mi-tosis. Here we show that, in response to DNA damage, Saccharomyces cerevisiae Scc3, one of the major regulatory subunits of the Smc1-Smc3-Scc1 cohesin ring, is phosphorylated on S/T-Q residues. This event depended on the Mec1 checkpoint kinase as well as on cell cycle arrest triggered by the DNA damage checkpoint network. This phosphorylation event also took place during mito-sis of an unperturbed cell cycle. The present finding that S. cerevisiae Scc3 is phosphorylated during mitosis represents a potentially important new regulatory step in cohesin's mitotic functions.
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Nathalie Grandin, Michel Charbonneau. Mec1-Dependent Phosphorylation of the Scc3 Subunit of Cohesin during Mitosis in Budding Yeast. Advances in Bioscience and Biotechnology, 2015, 06 (03), pp.153-163. ⟨10.4236/abb.2015.63015⟩. ⟨hal-01154292⟩

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