Mec1-Dependent Phosphorylation of the Scc3 Subunit of Cohesin during Mitosis in Budding Yeast

Abstract : Cohesin is an evolutionary conserved complex that controls chromosome segregation during mi-tosis. Here we show that, in response to DNA damage, Saccharomyces cerevisiae Scc3, one of the major regulatory subunits of the Smc1-Smc3-Scc1 cohesin ring, is phosphorylated on S/T-Q residues. This event depended on the Mec1 checkpoint kinase as well as on cell cycle arrest triggered by the DNA damage checkpoint network. This phosphorylation event also took place during mito-sis of an unperturbed cell cycle. The present finding that S. cerevisiae Scc3 is phosphorylated during mitosis represents a potentially important new regulatory step in cohesin's mitotic functions.
Type de document :
Article dans une revue
Advances in Bioscience and Biotechnology, 2015, 06 (03), pp.153-163. 〈10.4236/abb.2015.63015〉
Liste complète des métadonnées

Littérature citée [27 références]  Voir  Masquer  Télécharger

https://hal-univ-tours.archives-ouvertes.fr/hal-01154292
Contributeur : Michel Charbonneau <>
Soumis le : jeudi 21 mai 2015 - 15:17:29
Dernière modification le : mercredi 21 mars 2018 - 10:54:03
Document(s) archivé(s) le : jeudi 20 avril 2017 - 06:22:31

Fichier

ABB_2015031715421497.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Nathalie Grandin, Michel Charbonneau. Mec1-Dependent Phosphorylation of the Scc3 Subunit of Cohesin during Mitosis in Budding Yeast. Advances in Bioscience and Biotechnology, 2015, 06 (03), pp.153-163. 〈10.4236/abb.2015.63015〉. 〈hal-01154292〉

Partager

Métriques

Consultations de la notice

198

Téléchargements de fichiers

60