Comparison of structure and organization of cutaneous lipids in a reconstructed skin model and human skin: spectroscopic imaging and chromatographic profiling.

Abstract : The use of animals for scientific research is increasingly restricted by legislation, increasing the demand for human skin models. These constructs present comparable bulk lipid content to human skin. However, their permeability is significantly higher, limiting their applicability as models of barrier function, although the molecular origins of this reduced barrier function remain unclear. This study analyses the stratum corneum (SC) of one such commercially available reconstructed skin model (RSM) compared with human SC by spectroscopic imaging and chromatographic profiling. Total lipid composition was compared by chromatographic analysis (HPLC). Raman spectroscopy was used to evaluate the conformational order, lateral packing and distribution of lipids in the surface and skin/RSM sections. Although HPLC indicates that all SC lipid classes are present, significant differences are observed in ceramide profiles. Raman imaging demonstrated that the RSM lipids are distributed in a non-continuous matrix, providing a better understanding of the limited barrier function.
Liste complète des métadonnées

https://hal-univ-tours.archives-ouvertes.fr/hal-01128914
Contributeur : Martin Soucé <>
Soumis le : mardi 10 mars 2015 - 15:40:02
Dernière modification le : mercredi 29 novembre 2017 - 09:36:57

Identifiants

Collections

Citation

Ali Tfayli, Franck Bonnier, Zeineb Farhane, Danielle Libong, Hugh J Byrne, et al.. Comparison of structure and organization of cutaneous lipids in a reconstructed skin model and human skin: spectroscopic imaging and chromatographic profiling.. Experimental Dermatology, Wiley, 2014, 23 (6), pp.441-443. 〈http://onlinelibrary.wiley.com/doi/10.1111/exd.12423/abstract〉. 〈10.1111/exd.12423〉. 〈hal-01128914〉

Partager

Métriques

Consultations de la notice

31