Comparison of structure and organization of cutaneous lipids in a reconstructed skin model and human skin: spectroscopic imaging and chromatographic profiling.

Ali Tfayli 1, * Franck Bonnier 2, 3 Zeineb Farhane 1 Danielle Libong 1 Hugh J Byrne 2 Arlette Baillet-Guffroy 1
* Corresponding author
1 EA4041 Groupe de Chimie Analytique - Laboratoire d'Etudes des Techniques et Instruments d'Analyse Moléculaire
2 Focas Research Institute
3 NMNS - Nanomédicaments et Nanosondes, EA 6295
Abstract : The use of animals for scientific research is increasingly restricted by legislation, increasing the demand for human skin models. These constructs present comparable bulk lipid content to human skin. However, their permeability is significantly higher, limiting their applicability as models of barrier function, although the molecular origins of this reduced barrier function remain unclear. This study analyses the stratum corneum (SC) of one such commercially available reconstructed skin model (RSM) compared with human SC by spectroscopic imaging and chromatographic profiling. Total lipid composition was compared by chromatographic analysis (HPLC). Raman spectroscopy was used to evaluate the conformational order, lateral packing and distribution of lipids in the surface and skin/RSM sections. Although HPLC indicates that all SC lipid classes are present, significant differences are observed in ceramide profiles. Raman imaging demonstrated that the RSM lipids are distributed in a non-continuous matrix, providing a better understanding of the limited barrier function.
Keywords : lipids human skin artificial skin models Raman spectroscopy stratum corneum high-performance liquid chromatography
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Journal articles
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https://hal-univ-tours.archives-ouvertes.fr/hal-01128914
Contributor : Martin Soucé <>
Submitted on : Tuesday, March 10, 2015 - 3:40:02 PM
Last modification on : Wednesday, November 13, 2019 - 12:22:06 PM

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Ali Tfayli, Franck Bonnier, Zeineb Farhane, Danielle Libong, Hugh J Byrne, et al.. Comparison of structure and organization of cutaneous lipids in a reconstructed skin model and human skin: spectroscopic imaging and chromatographic profiling.. Experimental Dermatology, Wiley, 2014, 23 (6), pp.441-443. ⟨10.1111/exd.12423⟩. ⟨hal-01128914⟩

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